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Nature Methods features HeLa proteome study from Liu Lab

“We are proteomic scientists and therefore have a long-term research interest in reproducibility and variability analysis,” says Liu. In 2014, they had the idea of using mass spectrometry to profile the proteomic heterogeneity in cell lines with the “same name.” To this end, they used ‘sequential-window acquisition of all theoretical fragments’ mass spectrometry (SWATH-MS), which can efficiently and reproducibly detect thousands of proteins in cell samples.

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