The Liu lab has published a pSILAC-DIA method for measuring protein turnover at EMBO Molecular Systems Biology (IF=9.8). Profiling of biological relationships between different molecular layers dissects regulatory mechanisms that ultimately determine cellular function. To thoroughly assess the role of protein post‐translational turnover, we devised a strategy combining pulse stable isotope‐labeled amino acids in cells (pSILAC), data‐independent acquisition mass spectrometry (