Three Lolly Pops

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pSILAC-DIA for measuring protein turnover with DIA-MS

The Liu lab has published a pSILAC-DIA method for measuring protein turnover at EMBO Molecular Systems Biology (IF=9.8). Profiling of biological relationships between different molecular layers dissects regulatory mechanisms that ultimately determine cellular function. To thoroughly assess the role of protein post‐translational turnover, we devised a strategy combining pulse stable isotope‐labeled amino acids in cells (pSILAC), data‐independent acquisition mass spectrometry (DIA‐MS), and a novel data analysis framework that resolves protein degradation rate on the level of mRNA alternative splicing isoforms and isoform groups. We demonstrated our approach by the genome‐wide correlation analy