Recent Posts

Archive

Tags

GenomeWeb features the recent DIA-MS study from Liu Lab


https://www.genomeweb.com/proteomics-protein-research/yale-team-combines-dia-ms-crispr-study-down-syndrome-protein

Using two-hour DIA mass spec runs on a Thermo Fisher Scientific Orbitrap Lumos instrument, the researchers were able to reproducibly quantify roughly 6,200 proteins in HeLa cells with CRISPR-deleted HMGN1, confirming deletion of the target gene and identifying 147 proteins with expression levels that changed significantly following knockout of this gene.

According to Yansheng Liu, assistant professor of pharmacology at Yale and senior author on the study, the paper offers a proof-of-concept that DIA mass spec can be a rapid and effective method for confirming the outcome of CRISPR experiments while also assessing the downstream effects of the targeted genetic alterations.

Please see our 2019 Proteomics paper here: https://onlinelibrary.wiley.com/doi/abs/10.1002/pmic.201800438